An mRNA vaccine elicits STING-dependent antitumor immune responses

Lipid-formulated RNA vaccines have been widely used for disease prevention and treatment, yet their mechanism of action and individual components contributing to such actions remain to be delineated. Here, we show that a therapeutic cancer vaccine composed of a protamine/mRNA core and a lipid shell is highly potent in promoting cytotoxic CD8+ T cell responses and mediating anti-tumor immunity. Mechanistically, both the mRNA core and lipid shell are needed to fully stimulate the expression of type I interferons and inflammatory cytokines in dendritic cells. Stimulation of interferon-β expression is exclusively dependent on STING, and antitumor activity from the mRNA vaccine is significantly compromised in mice with a defective Sting gene. Thus, the mRNA vaccine elicits STING-dependent antitumor immunity.

Mechanisms underlying microRNA-125a-mediated inhibition of proliferation of HaCaT cells by targeting the interleukin 23 receptor signaling pathway: a preliminary study / 中华皮肤科杂志

Objective:To explore the mechanism underlying microRNA (miR) -125a-mediated inhibition of proliferation of keratinocytes.Methods:After 24-hour pretreatment with interleukin (IL) -23, human HaCaT keratinocytes were divided into miR-125a group and miR-NC group transfected with a miR-125a overexpression plasmid and a control plasmid, respectively. Cell counting kit-8 (CCK8) assay was performed to evaluate the proliferative ability of HaCaT cells in the two groups at 0, 24, 48 and 72 hours after transfection, real-time fluorescence-based quantitative PCR to determine the mRNA expression of miR-125a and IL-23 receptors (IL-23R) in the two groups 24 hours after transfection, and Western blot analysis to determine the protein expression of IL-23R, Janus kinase 2 (JAK2) , protein kinase B (AKT) and phosphorylated AKT (p-AKT) in the two groups 48 hours after transfection. Dual-luciferase reporter assay was performed to verify the targeting relationship between miR-125a and IL-23R. Comparison of means between two groups was carried out by using t test, and changes in the proliferative ability of HaCaT cells over time were evaluated by using repeated measures analysis of variance. Results:After plasmid transfection, the relative expression of miR-125a was significantly higher in the miR-125a group (6.377 ± 0.745) than in the miR-NC group (0.700 ± 0.222; t=7.305, P=0.002) . At 0, 24 and 48 hours after transfection, there was no significant difference in cellular proliferative ability between the miR-125a group and the miR-NC group ( t=0.663, 0.623 and 1.930, respectively, all P > 0.05) ; at 72 hours after transfection, the cellular proliferative ability was significantly lower in the miR-125a group than in the miR-NC group ( t=4.407, P < 0.05) . The IL-23R mRNA expression was significantly lower in the miR-125a group than in the miR-NC group ( t=3.082, P < 0.05) . Compared with the miR-NC group, the miR-125a group showed significantly decreased protein expression of IL-23R, JAK2 and p-AKT ( t=11.715, 6.996, 12.424, P < 0.001,=0.002, < 0.001, respectively) . Dual-luciferase reporter assay showed targeted binding of miR-125a to IL-23R. Conclusion:MiR-125a may inhibit the proliferation of keratinocytes by negatively regulating the IL-23R/JAK2/AKT signaling pathway.

Efficacy of 308-nm excimer laser versus high-intensity ultraviolet radiation for the treatment of active localized vitiligo / 中华皮肤科杂志

Objective To investigate the efficacy and safety of 308-nm excimer laser compared to high-intensity ultraviolet radiation for the treatment of active localized vitiligo,and to observe changes in skin lesions before and after the treatment by confocal laser scanning microscopy.Methods Sixty patients with 203 skin lesions of active localized vitiligo and were enrolled into this study,and the vitiligo disease activity (VIDA) score of these patients ranged from 2 to 3.We selected more than 3 skin lesions from a same anatomical site of each patient,one of lesions served as a control and the other skin lesions (≥ 2) were randomly treated with 308-nm excimer laser (laser group) or high-intensity ultraviolet radiation (ultraviolet group).The treatment was conducted twice a week for 25 sessions,and a 3-month follow-up was performed.Results A total of 48 patients with 169 skin lesions completed the trial.The marked response rate was significantly higher in the laser group [66.15％ (43/65)] than in the ultraviolet group [44.64％ (25/56),x2 =8.28,P ＜ 0.01].The patients with a VIDA score of 2 showed a significantly higher marked response rate [67.69％(44/65)] compared with those with a VIDA score of 3 [44.64％(25/56),x2 =6.80,P ＜ 0.01].During the 3-month follow-up,no relapse was observed.Confocal laser scanning microscopy showed that the number of inflammatory cells increased at the dermal-epidermal junction of the intra-and extra-marginal lesional skin.After treatment,the number of inflammatory cells markedly decreased and returned to normal level in lesions.Conclusion Both 308-nm excimer laser and high-intensity ultraviolet radiation are effective in the treatment of active localized vitiligo,but the 308-nm excimer laser shows a more rapid onset of action and a better therapeutic effect.

MRI diagnosis of tubal ovarian cysts / 实用放射学杂志

Objective To evaluate MRI features of tubal ovarian cysts,to improve the understanding of its imaging characters. Methods 1 5 patients with tubal ovarian cysts proved by pathology and surgery were analyzed retrospectively.Results Among the 1 5 cases with cysts,9 cases were solitary lesions,including the right side of the attachment area in 5 cases and the left side of the at-tachment area in 4 cases.6 cases were multiple lesions located on both sides.The diameters of these cysts were from 3.8 cm to 1 1.0 cm,and average diameter was 5.6 cm.All cases showed that the cysts were round,communicated with fallopian tube.All tubals showed different degrees of expansion.The signals of the cysts showed hypointense on T1 WI and hyperintense on T2 WI.The signals in two cases were complex.After contrast medium administration,the walls of the cysts could be enhanced with different de-grees.Conclusion MRI is useful in displaying the morphology,signal and the wall of the tubal ovarian cysts.

康复与康复工程 / 中国康复理论与实践

@#本文就康复概念的“泛化”现象 ,提出了一些看法。对康复科学、康复系统工程及其对工程技术的需求 ,进行了分析探讨。结论 :康复工程的广义内涵是理清边界、拾遗补缺、设置接口、实现系统集成 ,利用现代工程技术的手段和方法促进残疾者的全面康复。

Analysis on Construction of Male Urine Collection Device / 中国康复理论与实践

@#Objective: Finding the essence and regular pattern of the construction of male urine collection devices, and setting up its basic concepts. Methods:Selecting typical construction examples, then analyzing them singly and as a whole. Results:Setting up concepts such as construction module, construction factor, construction factor unit, construction index, construction array etc., and giving out the table of construction factor units. Conclusion:This research has provided mode, methods, means for understanding and further analyzing, studying male urine collection devices.

The Development of Braille Writing Board with Raised Braille Characters / 中国康复理论与实践

@#In this paper the development of a new Braille writing board on which raised Braille characters are written is introduced. Compared with traditional Braille writing board on which hollow Braille characters are written, the advantage of new one is convenient for studying, writing and proofreading because the order of Braille in writing is the same as the order of Braille in remembering.

康复工程的实践与理论 / 中国康复理论与实践

@#本文在系统观的指导下，归纳出康复工程发展的四个阶段，及其在康复领域中起到了不可取代的作用。进一步论述了康复系统工程、康复工程及其在康复体系中所处的地位。最后提出了，作为一项专门工程技术体系，康复工程在我国尚处于发展初期，需要拾遗补缺，突出特点，加强在界面／接口处作文章的观点。

中国残疾人用品用具工作现状 / 中国康复理论与实践

@#本文对当前我国残疾人用品用具总体工作的状况进行了扼要介绍和评述，包括政策与政府的主导作用、对用品用具的需求、产品开发生产与供应服务、专业人员教育与培训、国际交往与合作等。本文认为，残疾人用品用具作为一个行业在我国已初步形成，指出了该行业目前存在的几个问题。

Research on the Method of Quantitative Assessment for Walking Function / 中国康复理论与实践

@# In this paper a series of gait quantitative assessment indexes:phase symmetry index,verti-cal force symmetry index,equilibrium function index,and brake and drive function indexes are obtainedwith VICON 3-dimensinal movement analysis system according to the results that 40 amputees,50 cerebralpalsy patients, 31 hemiplegia patients and 19 paraplegia are evaluated and the differences between the nor-mal and the abnormal(p<0.001)found with these indexes. The quantitative assessment methed of walk-ing function not only provides rehabilitation level of patients and for checking therapeutical effects,but alsolaies the foundation of gait analysis to standardization and practicalization further.

A Dynamic EMG Study on Co-contraction of Quadriceps and Hamstrings in Spastic Hemiplegic Gait / 中国康复理论与实践

@#The purpose of this study was to propose a co-contraction profile(CCP)to assess co-con-traction of agonist and antagonist muscles about the knee in spastic hemiplegic gait.Surface EMG wasrecorded from quadriceps and hamstrings of 9 ablet-bodied subjects and 6 spastic hemiplegic subjects follow-ing stroke.Results showed that CCPs were high during weight bearing both in able-bodied subject walkingand in subjects with spastic hemiplegia. However, they were higher in most part of stance phase and lateswing phase in spastic hemiplegics compared to able-bodied subjects (P<0.05).The study concluded thatco-contraction profiles provide descriptive information on the sources of variation in co-contraction,particu-larly in co-contraction in assessment of spastic hemiparetic subjects following stroke.